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Journal / Immunology and Infection / Tek adımlı olumsuz Kromatografik arıtma
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Immunology and Infection

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JoVE Journal Immunology and Infection
Tek adımlı olumsuz Kromatografik arıtma
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Journal (Immunology and Infection)
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
Journal (Immunology and Infection)
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
 
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Tek adımlı olumsuz Kromatografik arıtma

Article DOI: 10.3791/54043-v • 10:44 min • June 18th, 2016
June 18th, 2016 •
1, 1, 1, 1, 1,2
1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

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Rekombinant Helicobacter tek-aşamalı negatif saflaştırılması için yüksek verimli bir yöntem tarif edilmektedir küme modunda dietilaminoetil reçineleri kullanılarak Escherichia coli içinde aşırı eksprese nötrofil aktive edici protein (HP-NAP) pylori. Bu yöntem ile arıtılmış, HP-NAP H. aşı, ilaç veya teşhis maddelerinin geliştirilmesi için faydalıdır pylori hastalıkları -associated.

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İmmünoloji Sayı 112 HP-NAP, DEAE Negatif kromatografisi bağlı olmayan fraksiyon Toplu kromatografisi,
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