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CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
JoVE Journal
Biology
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JoVE Journal Biology
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
DOI:

08:09 min

August 04, 2016

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Chapters

  • 00:05Title
  • 01:19Transparent Mouse Skin Tissue Preparation
  • 02:32Mouse Skin Biopsy Clearance
  • 04:27Mouse Skin Biopsy Imaging
  • 05:08Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations
  • 06:55Conclusion

Summary

Automatic Translation

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.

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