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Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
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Neuroscience
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JoVE Journal Neuroscience
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells

DOI:
10.3791/55359-v

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07:48 min

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August 27, 2017

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, , ,
1Department of Anatomy and Cell Biology,Martin Luther University Halle-Wittenberg, 2Department of Anatomy,University of Otago

Chapters

  • 00:00Title
  • 00:08Agar Preparation
  • 00:54Brain Fixation
  • 02:29Slicing Procedure
  • 03:26Hippocampus Removal
  • 04:05Culturing Method
  • 05:18Representative Results
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

Organotypic hippocampal slice cultures (OHSC) represent an in vitro model that simulates the in vivo situation very well. Here we describe a vibratome-based improved slicing protocol to obtain high quality slices for use in assessing the neuroprotective potential of novel substances or the biological behavior of tumor cells.

Tags

Organotypic Hippocampal Slice CulturesNeuroprotectionTumor Cell InvasivenessVibratomeAgar BlocksPreparation MediumCytoarchitectureCell Culture InsertCulture MediumInflammatory ReactionsNeuroprotection Studies

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