Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / En 5-mC primärblottanalys som kvantifierar…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
En 5-mC primärblottanalys som kvantifierar DNA-metyleringsnivån av kondrocyt-skillnad
Determining Bile Duct Density in the Mouse Liver
Journal (Developmental Biology)
Determining Bile Duct Density in the Mouse Liver
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
Journal (Developmental Biology)
Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane
 
Click here for the English version

En 5-mC primärblottanalys som kvantifierar DNA-metyleringsnivån av kondrocyt-skillnad

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi presenterar en metod för att kvantifiera DNA-metylering baserat på 5-metylcytosin (5-mC) dot blot. Vi bestämde 5-mC-nivåerna vid chondrocyt dedifferentiering. Denna enkla teknik kan användas för att snabbt bestämma kondrocytfenotypen vid ACI-behandling.

Tags

Utvecklingsbiologi utgåva 123 cellkultur monoskiktsexpansion kondrocytdifferentiering DNA-metylering 5-mC dot-blot-analys genomisk DNA-extraktion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter