Journal
/
Biology
/
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

DOI:
10.3791/55996-v

•

13:08 min

•

August 10, 2017

•

, , , ,
1School of Biological Sciences,Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing,National University of Singapore

Chapters

  • 00:05Title
  • 00:35Cell Seeding
  • 02:00Cell Transfection and Nocodazole Treatment
  • 02:50Image Acquisition of Multi-color Beads and Golgi Mini-stacks
  • 04:36Image Analysis, Acquire Centers of Fluorescent Beads
  • 07:57Select and Measure Golgi Mini-stacks
  • 10:59Results: Imaging the Golgi’s Center of Fluorescence Mass
  • 12:31Conclusion

Summary

Automatic Translation

Automatic Translation

The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.

Tags

Quantitative LocalizationGolgi ProteinFluorescence MassHeLa CellsTransfectionNocodazoleFluorescent BeadsImagingMicroscopy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Quantitative Assessment of The Yeast Lipidome using Electrospray Ionization Mass Spectrometry

Time-lapse Imaging of Mitosis After siRNA Transfection

Analyzing Large Protein Complexes by Structural Mass Spectrometry

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Rapid Analysis and Exploration of Fluorescence Microscopy Images

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Read Article