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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ MHC-tetramer farvning og kvantitativ analyse til at bestemme placering, overflod og fænotype af Antigen-specifikke CD8 T celler i væv
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In Situ MHC-tetramer farvning og kvantitativ analyse til at bestemme placering, overflod og fænotype af Antigen-specifikke CD8 T celler i væv

Article DOI: 10.3791/56130-v • 08:37 min • September 22nd, 2017
September 22nd, 2017 •
1, 1, 1
1Department of Veterinary and Biomedical Sciences, University of Minnesota

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Her, beskriver vi en metode, der kombinerer i situ MHC-tetramer farvning med Immunhistokemi at bestemme lokalisering, fænotype og mængden af antigen-specifikke T-celler i væv. Denne protokol bruges til at bestemme de rumlige og fænotypiske egenskaber af antigen-specifikke CD8 T celler i forhold til andre celletype og strukturer i væv.

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Immunologi sag 127 In situ tetramer farvning (IST) virus-specifikke CD8 + T celler immunhistokemi lokalisering fænotype Konfokal mikroskopi kvantitative billedanalyse
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