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Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
DOI:

11:33 min

August 17, 2017

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Chapters

  • 00:05Title
  • 00:37Phage Amplification
  • 02:15Purification of Phage
  • 05:39Double-stranded Genome DNA Extraction
  • 08:19Cell-free Phage Reaction and Phage Titer Experiment
  • 09:35Results: Synthesis and Quantification of Phage T7 in Varying Molecular-crowding Environments
  • 10:46Conclusion

Summary

Automatic Translation

A new generation of cell-free transcription-translation platforms has been engineered to construct biochemical systems in vitro through the execution of gene circuits. In this article, we describe how bacteriophages, such as MS2, ΦΧ174, and T7, are synthesized from their genome using an all E. coli cell-free TXTL system.

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