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In Vitro Ensayo de la sumoilación estudiar SUMO E3 ligasa actividad
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In Vitro Ensayo de la sumoilación estudiar SUMO E3 ligasa actividad

Article DOI: 10.3791/56629-v • 09:45 min • January 29th, 2018
January 29th, 2018 •
1, 2, 2,3,4,5, 1,6
1Institute of Microbiology and Immunology, National Yang-Ming University, 2UC Davis Cancer Center, University of California, Davis, 3Department of Biochemistry and Molecular Medicine, University of California, Davis, 4Institute for Translational Medicine, College of Medical Science and Technology, Taipei Medical University, 5Division of Molecular and Genomic Medicine, National Health Research Institutes, 6Center for Infectious Disease and Cancer Research, Kaohsiung Medical University

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A diferencia de ligasas de ubiquitina ligasas E3 SUMO algunos han sido identificados. Este modificado en vitro protocolo de sumoilación es capaz de identificar nuevos SUMO E3 ligasas por un ensayo de reconstitución en vitro .

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Biología molecular número 131 In vitro la sumoilación K-bZIP SUMO E3 ligasa p53 modificación poste-de translación Ubc9
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