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A Protocol for Real-time 3D Single Particle Tracking
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Bioengineering
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JoVE Journal Bioengineering
A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

DOI:
10.3791/56711-v

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10:16 min

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January 03, 2018

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1Department of Chemistry,Duke University

Chapters

  • 00:05Title
  • 00:31Overview of the Apparatus
  • 01:44Preparing the Samples
  • 02:21Optimizing the Tracking Parameters
  • 07:12Real-time Tracking of Freely Diffusing Nanoparticles
  • 08:52Results: Recreating the Path of A Freely Diffusing Nanoparticle
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol details the construction and operation of a real-time 3D single particle tracking microscope capable of tracking nanoscale fluorescent probes at high diffusive speeds and low photon count rates.

Tags

3D Single Particle TrackingReal-time MicroscopyVirology488 Nm LaserElectro-optic DeflectorsTunable Acoustic Gradient LensAvalanche PhotodiodeFPGAFluorescent BeadsPhosphate Buffered SalineWaterNano PositionerPhoto DetectorFocal PlaneRaster Scan

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