Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Met behulp van 3 Single-molecuul FRET te…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
Met behulp van 3 Single-molecuul FRET te bestuderen de correlatie van eiwitinteractie
Extraction and Analysis of Taiwanese Green Propolis
Journal (Biochemistry)
Extraction and Analysis of Taiwanese Green Propolis
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
Journal (Biochemistry)
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
 
Click here for the English version

Met behulp van 3 Single-molecuul FRET te bestuderen de correlatie van eiwitinteractie

Article DOI: 10.3791/56896-v • 11:22 min • January 30th, 2018
January 30th, 2018 •
1, 1, 1,2, 1
1Institute of Physical Chemistry, University of Freiburg, 2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier presenteren we een protocol om gegevens van de drie kleuren smFRET en haar analyse met een 3D ensemble verborgen Markov Model te verkrijgen. Met deze benadering kunnen wetenschappers kinetische informatie ophalen uit complexe eiwit systemen, met inbegrip van Allosterie of gecorreleerde interacties.

Tags

Biochemie Biofysica kwestie 131 eiwit kinetiek Multi-Color smFRET TIRF HMM Hsp90 Allosterie single-molecuul FRET
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter