00:49Fluorescence Labeling of Extracellular Vesicles
03:05Visualize Uptake of Extracellular Vesicles by Confocal Microscopy
06:18Endothelial Cell Permeability
07:36Determine Puromycin Sensitivity
08:59Transduction of Endothelial Cells with Lentiviral Vector
10:53Results: Use of Confocal Microscopy and Diffusion Capacity of Fluorochrome Dextran to Study the Interaction Between the Extracellular Vesicles and Endothelial Cells
In this work, we describe protocols to investigate the role of extracellular vesicles (EVs) released by Plasmodium falciparum infected erythrocytes. In particular, we focus on the interactions of EVs with endothelial cells.