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A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
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JoVE Journal Biology
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

DOI:
10.3791/57258-v

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09:22 min

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January 25, 2018

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1Department of Biology,California State University, Northridge

Chapters

  • 00:05Title
  • 00:57Protoplast Isolation
  • 04:09Protoplast Transformation
  • 06:00Protoplast Incubation and Harvesting
  • 06:46Results: Efficient Isolation of Soybean Protoplasts for Transient Gene Expression Studies
  • 08:28Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a simple and efficient protocol for the preparation of large quantities of soybean protoplasts to study complex regulatory and signaling mechanisms in live cells.

Tags

Soybean ProtoplastsTransient Gene ExpressionRegulatory NetworksProtein InteractionsUnifoliate LeavesEnzyme SolutionVacuum InfiltrationProtoplast DigestionW5 SolutionNylon MeshCentrifugation

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