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Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains
DOI:

09:44 min

July 10, 2018

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Chapters

  • 00:04Title
  • 00:40Preparing the Microinjection Apparatus
  • 04:09Microinjecting Neonatal Mice
  • 07:30Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum
  • 09:03Conclusion

Summary

Automatic Translation

We describe a protocol of stereotaxic surgery with a homemade head-fixed device for microinjecting reagents into the striatum of neonatal mouse brains. This technique allows genetic manipulation in neuronal cells of specific regions of neonatal mouse brains.

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