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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR を介した損失関数における小脳顆粒細胞のエレクトロポレーション法による遺伝子導入の子宮内を使用して
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CRISPR を介した損失関数における小脳顆粒細胞のエレクトロポレーション法による遺伝子導入の子宮内を使用して

Article DOI: 10.3791/57311-v • 09:39 min • June 9th, 2018
June 9th, 2018 •
*1, *2, 2, 3,4,5, 1, 3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
* These authors contributed equally

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ノックアウト動物を用いた遺伝子の機能喪失従来は、高価で時間のかかる多くの場合されています。CRISPR 媒介体細胞突然変異誘発のエレクトロポレーション ベースは遺伝子の生体内の機能を理解するための強力なツールです。小脳の細胞増殖にノックアウトの表現型を解析する手法を報告する.

Tags

発生生物学、問題 136、ターゲット遺伝子デリバリー、子宮内エレクトロポレーション、小脳、GNP、Cre、CRISPR/Cas9、脳の発達、脳腫瘍
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