Journal
/
Developmental Biology
/
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

DOI:
10.3791/57330-v

•

08:55 min

•

April 25, 2018

•

,
1Department of Comparative Pathobiology,Purdue University, 2Purdue University Center for Cancer Research, Purdue Institute for Inflammation, Immunology and Infectious Diseases (PI4D), Purdue Institute for Integrative Neuroscience (PIIN),Purdue University

Chapters

  • 00:05Title
  • 00:46Microinjection
  • 05:24Imaging
  • 06:52Results: Visualization of Embryonic Development, Movement, and Tumor Cells In Vivo
  • 08:11Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we show the process of creating a cellular electric voltage reporter zebrafish line to visualize embryonic development, movement, and fish tumor cells in vivo.

Tags

ZebrafishEmbryogenesisCellular Electrical ActivityTumorIn VivoReal-time ImagingTol2 TransposaseMRNA InjectionEmbryo CollectionAgarose Injection MoldMicroinjection

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Mechanical Vessel Injury in Zebrafish Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Isolation and Characterization of Single Cells from Zebrafish Embryos

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Read Article