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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
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Bioengineering
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JoVE Journal Bioengineering
Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

DOI:
10.3791/57474-v

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11:37 min

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June 18, 2018

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, , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,Rostock University Medical Center, 2Department Life, Light and Matter of the Interdisciplinary Faculty,Rostock University

Chapters

  • 00:04Title
  • 00:53CD133 SC Isolation
  • 06:00Characterization of Freshly Isolated CD133 SCs
  • 07:21Preparation of Transfection Complexes
  • 08:26CD133 SCs Transfection and Analysis
  • 10:03Results: Successful Engineering of miR-loaded Magnetized CD133 Cells
  • 11:02Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging.

Tags

MicroRNA TransferAdult Bone Marrow-derived Hematopoietic Stem CellsCell EngineeringMagnetic TargetingRegenerative MedicineHematopoietic Stem Cell ModificationMesenchymal Stem CellsBone Marrow CollectionDensity Gradient CentrifugationMononuclear Cell IsolationMagnetic Selection

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