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Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
DOI:

12:36 min

June 26, 2018

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Chapters

  • 00:04Title
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

Automatic Translation

Here we describe a single-cell proteomic approach to evaluate immune phenotypic and functional (intracellular cytokine induction) alterations in peripheral whole blood samples, analyzed via mass cytometry.

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