Journal
/
Developmental Biology
/
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
JoVE Journal
Developmental Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

DOI:
10.3791/57784-v

•

06:17 min

•

August 25, 2018

•

, , , , , ,
1Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology,Shandong University, 2Suzhou Institute of Shandong University, 3Department of Urology,Qilu Hospital of Shandong University

Chapters

  • 00:00Title
  • 00:20Skin Tissue Pretreatment
  • 01:07Digestion of skin tissue
  • 02:11Collecting and culturing primary cells
  • 03:42Cell Passage
  • 04:46Representative results
  • 06:06Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

Tags

Primary Human KeratinocytesIsolationCultureSimplified MethodEfficientEpidermal CellsLaboratory ApplicationsClinical ApplicationsHomogenizationEnzymatic DigestionTrypsinDNase ICell StrainerCell CultureROCK InhibitorKeratinocyte MediumPassaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

An Efficient Method to Obtain Dedifferentiated Fat Cells

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Generation and Culturing of Primary Human Keratinocytes from Adult Skin

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

A Direct and Simple Method to Assess Drosophila melanogaster‘s Viability from Embryo to Adult

Read Article