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Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat
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Neuroscience
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JoVE Journal Neuroscience
Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat

Simple Surgical Induction of Conductive Hearing Loss with Verification Using Otoscope Visualization and Behavioral Clap Startle Response in Rat

DOI:
10.3791/57993-v

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00:06 min

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October 26, 2019

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, , , , , ,
1Instituto de Neurobiología,Universidad Nacional Autónoma de México, México, 2State Key Laboratory of Marine Pollution (SKLMP),City University of Hong Kong, Hong Kong, 3Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences,City University of Hong Kong, Hong Kong, 4Department of Physics,City University of Hong Kong, Hong Kong, 5Department of Clinical Oncology,Queen Elizabeth Hospital, Hong Kong, 6Department of Materials Science and Engineering,City University of Hong Kong, Hong Kong

Chapters

  • 00:00Title
  • 01:05Animals
  • 01:36Surgical Setup
  • 02:18Surgical Induction of Conductive Hearing Loss
  • 02:55Otoscope Visualization
  • 03:47Behavioral Assessment
  • 04:38Failure to Induce CHL
  • 05:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to establish a replicable conductive hearing loss induction via surgical tympanic membrane puncture and verification by otoscope visualization and behavioral assessment by clap startle.

Tags

Surgical InductionConductive Hearing LossOtoscope VisualizationBehavioral Clap Startle ResponseRat ModelTympanic Membrane PunctureMalleus RemovalAnesthesiaSurgical ProcedureEthical Approval

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