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In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice
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JoVE Journal Neuroscience
In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

DOI:
10.3791/58340-v

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06:24 min

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October 18, 2018

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, ,
1International Research Center for Medical Sciences (IRCMS),Kumamoto University, 2Division of Neurogenetics,National Institute of Genetics, 3Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Chapters

  • 00:04Title
  • 00:33Exposing the Skull
  • 01:29Cranial Window Preparation
  • 03:29Two-Photon Imaging
  • 04:59Results: In vivo Two-Photon Imaging of Cortical Neurons in Neonatal Mice
  • 05:57Conclusion

Summary

Automatic Translation

Automatic Translation

We present an in vivo two-photon imaging protocol for imaging the cerebral cortex of neonatal mice. This method is suitable for analyzing the developmental dynamics of cortical neurons, the molecular mechanisms that control the neuronal dynamics, and the changes in neuronal dynamics in disease models.

Tags

In VivoTwo-photon ImagingCortical NeuronsNeonatal MiceNeuroscienceNeuron MorphologyCranial WindowAnesthesiaCraniotomyAgarose GelCover SlipDental CementTitanium BarAnalgesic

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