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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
DOI:

09:12 min

January 18, 2019

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Chapters

  • 00:04Title
  • 00:47Surgical Procedure
  • 03:00In Vivo Biotinylation and Glomeruli Isolation
  • 05:29Glomeruli Washing and Quantification
  • 06:25Results: Representative In Vivo Labeling of Glomerular Cell Surface Proteins
  • 08:31Conclusion

Summary

Automatic Translation

Here we present a protocol for murine in vivo labeling of glomerular cell surface proteins with biotin. This protocol contains information on how to perfuse mouse kidneys, isolate glomeruli, and perform endogenous immunoprecipitation of the protein of interest.

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