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Journal / Biochemistry / Nitropeptide Profilazione e identificazione…
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Biochemistry

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JoVE Journal Biochemistry
Nitropeptide Profilazione e identificazione illustrata da Angiotensin II
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
Journal (Biochemistry)
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
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Journal (Biochemistry)
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Nitropeptide Profilazione e identificazione illustrata da Angiotensin II

Article DOI: 10.3791/59391-v • 07:31 min • June 16th, 2019
June 16th, 2019 •
1,2, 3, 2,4,5,6
1Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 2Department of Biological Sciences, University of Notre Dame, 3Department of Urology, Shanghai East Hospital, Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases, University of Notre Dame, 5Harper Cancer Research Institute, University of Notre Dame, 6Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center

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La profilazione proteomica delle proteine tirosine-nitrirate è stata una tecnica impegnativa a causa della bassa abbondanza della modifica 3-nitrotyrosine. Qui descriviamo un nuovo approccio per l'arricchimento e la profilazione dei nitropeptidi usando Angiotensin II come modello. Questo metodo può essere esteso per altri sistemi in vitro o in vivo.

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Biochimica Numero 148 Nitropeptide Angiotensina II nitrotyrosine etichettatura dimetilla dithionate di sodio etichettatura biotina arricchimento
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