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A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
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Bioengineering
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JoVE Journal Bioengineering
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

DOI:
10.3791/60538-v

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09:12 min

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December 13, 2019

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, ,
1Center for Nanomedicine and Theranostics,Technical University of Denmark, 2Center for Intestinal Absorption and Transport of Biopharmaceuticals,Technical University of Denmark, 3Department of Health Technology,Technical University of Denmark

Chapters

  • 00:04Title
  • 00:58Liposome Preparation
  • 02:14Surface Preparation of Imaging Chamber
  • 02:57Liposome Immobilization
  • 03:42Image Acquisition and Data Analysis
  • 05:21Liposome Size Calibration
  • 06:42Results: Liposome Surface Immobilization and Compositional Inhomogeneity
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the fabrication of liposomes and how these can be immobilized on a surface and imaged individually in a massive parallel manner using fluorescence microscopy. This allows for the quantification of the size and compositional inhomogeneity between single liposomes of the population.

Tags

Single LiposomeLiposome CompositionLiposome InhomogeneityFluorescence MicroscopyLipid LabelingLipid MixingFreeze-thawExtrusionBSA CoatingStreptavidinSingle-molecule Detection

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