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Chapters
- 00:05Introduction
- 00:45P. falciparum Gametocyte Culture
- 02:23Standard Membrane Feeding Assay (SMFA)
- 04:57Midgut Dissection and Oocyst Load Quantification
- 06:01Salivary Gland Dissection and Sporozoite Load Quantification
- 07:00Results: Representative Gametocyte Culture Development
- 08:42Conclusion
Detailed investigations on mosquito stages of malaria parasites are critical to design effective transmission blocking strategies. This protocol demonstrates how to effectively culture infectious gametocytes and then feed these gametocytes to mosquitoes to generate mosquito stages of P. falciparum.
