Journal
/
/
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Actin Co-Sedimentation Assay; for the Analysis of Protein Binding to F-Actin
DOI:

07:53 min

March 28, 2008

,

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:44Preparation of Actin Filaments
  • 02:49Preparation of Protein and Binding of Actin
  • 04:26Running the Gel
  • 06:58Conclusion

Summary

Automatic Translation

Proteins bind to filamentous actin (F-actin) through distinct actin binding modules. In this video we demonstrate the procedure of actin co-sedimentation, which is an in vitro assay routinely used to analyze proteins or specific domains that bind F-actin.

Related Videos

Read Article