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Chapters
- 00:00Title
- 00:43Introduction
- 01:12Linearization of the Bradford Protein Calibration Graph
- 02:55Measuring Protein Concentration Using the Improved Bradford Protein Assay
- 04:59Improved Bradford Protein Assay Representative Results
- 05:53Conclusion
The accuracy and sensitivity of protein determination by the rapid and convenient Bradford assay is compromised by intrinsic nonlinearity. We show a simple linearization procedure that greatly increases the accuracy, improves the sensitivity of the assay about 10-fold, and significantly reduces interference by detergents.
