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Linearization of the Bradford Protein Assay

Linearization of the Bradford Protein Assay

Article DOI: 10.3791/1918
April 12th, 2010


Summary April 12th, 2010

The accuracy and sensitivity of protein determination by the rapid and convenient Bradford assay is compromised by intrinsic nonlinearity. We show a simple linearization procedure that greatly increases the accuracy, improves the sensitivity of the assay about 10-fold, and significantly reduces interference by detergents.

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