Biology
A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.
Summary April 12th, 2010
The accuracy and sensitivity of protein determination by the rapid and convenient Bradford assay is compromised by intrinsic nonlinearity. We show a simple linearization procedure that greatly increases the accuracy, improves the sensitivity of the assay about 10-fold, and significantly reduces interference by detergents.