Congelación de nematodos: un método para exponer los tejidos de gusano interiores para la tinción

Published: April 30, 2023

Abstract

Fuente: Zhang, N., et al. The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Anticuerpo Staining, RNAi Loss-of-function Analysis and Imaging. J. Vis. Exp. (2017).

Este video describe el liongelante, un método para hacer que los tejidos C. elegans sean accesibles para la tinción de anticuerpos alterando la cutícula.

Protocol

Este protocolo es un extracto de Zhang et al, The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging, J. Vis. Exp. (2017). Tinción de anticuerpos del intestino C. elegans fijación Tome una diapositiva de vidrio limpia y use poli-L-lisi…

Materials

Antibody staining
poly-L-lysine Sigma P5899
Methanol Fisher Scientific A452-4
Acetone Fisher Scientific A949SK-4
Permount Fisher Scientific SP15-100
Microscope slides Fisher Scientific 4448
Microscope coverslips (22×22-1) Fisher Scientific 12-542-B
M9 Medium lab made
OP50 bacteria CGC

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Cite This Article
Freeze-Cracking of Nematodes: A Method to Expose Interior Worm Tissues for Staining. J. Vis. Exp. (Pending Publication), e20130, doi: (2023).

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