Creation of micro-tissues using cylindrical collagen gels, called modules, that contain embedded cells and which surface is coated with endothelial cells.
We have fabricated several different micro-tissues using modules embedded with different cell types1-11. We have successfully embedded primary cardiomyocytes, islets, adipose stem cells and mesenchymal stromal cells as well as several cell lines including HepG2, NIH 3T3 and clone 9 liver cells. We have coated the modules with several types of endothelial cells including rat aortic endothelial cells, human umbilical vein endothelial cells and human microvascular endothelial cells. Modules have also been prod…
The authors have nothing to disclose.
Material Name | Type | Company | Catalogue Number | Comment |
---|---|---|---|---|
Intramedic tubing PE60 | Becton Dickson | 427416 | Different diameter of tubing can be used to change the diameter of the modules | |
Phosphate-buffered saline (PBS) | Gibco | 20012-027 | ||
Trypsin-EDTA | Gibco | 25200-072 | ||
Purcol acidificed collagen, 3 mg/mL | Cedarlane | 5005-B | ||
Sodium bicarbonate | Sigma-Aldrich | S5761 | ||
20G needle | Becton Dickson | 305175 | Diameter of the needle needs to be similar to the diameter of the tubing | |
3 mL syringe | BD Biosciences | 309585 | ||
15 mL tube | BD Biosciences | 352096 | ||
50 mL tube | BD Biosciences | 352070 | ||
Convertors Self-Seal Pouch 7 1/2” x 13” | Cardinal Health | 92713 | ||
10 ml Wide Tip serological pipet | BD Falcon | 357504 | ||
10 ml serological pipet | BD Falcon | 357551 | ||
5 ml serological pipet | BD Falcon | 357543 |