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主电穿孔的小鼠骨髓造血细胞培养制备
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Please note that all translations are automatically generated.
Click here for the English version.
主电穿孔的小鼠骨髓造血细胞培养制备
DOI:
10.3791/1026-v
•
08:15 min
•
January 06, 2009
•
Kelly Kroeger
,
Michelle Collins
,
Luis Ugozzoli
1
Gene Expression Division
,
Bio-Rad Laboratories, Inc
Chapters
00:00
Title
00:08
Introduction
00:43
Harvesting Bone Marrow From Femurs and Tibiae
02:07
Establishing Cultures
04:33
Electroporating Cells
07:13
Transfection Results
07:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
此过程介绍了如何建立从小鼠骨髓造血的主要细胞培养和使用转基因脉冲MXCell电系统。
Tags
Primary Hematopoietic Cell Cultures
Murine Bone Marrow
Electroporation
Plasmid DNA
SiRNA
Gene Pulser MXcell Electroporation System
Gene Pulser Electroporation Buffer
Transfection
Nucleic Acids
Mammalian Cells
Difficult-to-transfect Cells
Stem Cells
Video Demonstration
Femur
Tibia
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