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显微注射mRNA和斑马鱼的胚胎吗啉反义寡核苷酸。
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Biology
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
Please note that all translations are automatically generated.
Click here for the English version.
显微注射mRNA和斑马鱼的胚胎吗啉反义寡核苷酸。
DOI:
10.3791/1113-v
•
11:33 min
•
May 07, 2009
•
Shiaulou Yuan
,
Zhaoxia Sun
1
Department of Genetics
,
Yale University School of Medicine
Chapters
00:00
Title
00:54
Introduction
01:29
Preparation of RNA
03:26
Preparation of Morpholino
05:07
Preparing the Micropipette and Plates for Microinjections
07:51
Preparing and Injecting Fertilized Zebrafish Embryos
10:09
Representative Results: EGFP Expression and Mutant Phenotypes
11:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
显微注射是外来物质引入斑马鱼胚胎受精的完善而有效的方法。在这里,我们展示了一个强大的执行mRNA表达显微注射技术,和吗啉代寡核苷酸在斑马鱼的基因敲除研究。
Tags
Microinjection
MRNA
Morpholino Antisense Oligonucleotides
Zebrafish Embryos
Gene Knockdown
Overexpression Studies
Misexpression Studies
Gene Function
In Vivo Assay
EGFP MRNA
Translational-blocking Morpholino Oligo
Autosomal Dominant Polycystic Kidney Disease (ADPKD)
1-cell Stage Embryos
GFP Expression
Phenotype Analysis
Transgenic Animals
Germ-line Chimeras
Cell-fate Mapping
Gene Screening
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