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斑马鱼整装高分辨率双荧光原位杂交
JoVE Journal
Biology
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JoVE Journal Biology
Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
DOI:

12:31 min

March 25, 2009

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Chapters

  • 00:00Title
  • 01:00Introduction
  • 01:27Fixing and Dechorionating Embryos
  • 03:10Permeabilizing the embryos
  • 03:51Hybridization
  • 05:37Antibody labeling
  • 09:10Propidium iodide staining and detection
  • 10:31Outcome and Results
  • 11:41Conclusion

Summary

Automatic Translation

整装原位杂交是发育生物学中最广泛使用的技术之一。在这里,我们提出一个高分辨率的双荧光原位杂交协议分析一个单一的基因精确的表达模式,并确定两个基因的表达域重叠。我们包括核反击一个碘化丙啶染色,以突出组织机构。

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