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对于斑马鱼精子超低温冷冻保存的一个高通量的方法和<em在体外施肥
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
A High-Throughput Method For Zebrafish Sperm Cryopreservation and
In Vitro
Fertilization
Please note that all translations are automatically generated.
Click here for the English version.
对于斑马鱼精子超低温冷冻保存的一个高通量的方法和<em在体外施肥
DOI:
10.3791/1395-v
•
11:01 min
•
July 06, 2009
•
Bruce W. Draper
,
Cecilia B. Moens
1
Molecular and Cellular Biology
,
University of California, Davis
,
2
Division of Basic Science
,
Fred Hutchinson Cancer Research Center – FHCRC
Chapters
00:05
Title
00:08
Introduction
01:02
Sperm Freezing Solutions and Materials
02:04
Sperm Freezing Method
06:50
In Vitro Fertilization Method Using Cryopreserved Sperm
09:31
Representative Results/ Outcome
09:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
这是一种高通量的精子冷冻保存对斑马鱼的协议。精子冷冻保存,使用此协议,有25%在随后的体外受精生育的平均多年来保持稳定。
Tags
Zebrafish Sperm Cryopreservation
In Vitro Fertilization
High-throughput Method
Harvey Method Adaptation
Sample Uniformity
Freezing Media
Cryoprotectant
Cryovials
Sperm Freezing
Sperm Thawing
Capillary Tubes
Team Work
Fertility Rate
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