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Biology
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为介观荧光体层摄影术
在体内
成像的发展
果蝇</em
JoVE Journal
Biology
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JoVE Journal
Biology
Mesoscopic Fluorescence Tomography for
In-vivo
Imaging of Developing
Drosophila
Please note that all translations are automatically generated.
Click here for the English version.
为介观荧光体层摄影术
在体内
成像的发展
果蝇</em
DOI:
10.3791/1510-v
•
11:51 min
•
August 20, 2009
•
Claudio Vinegoni
,
Daniel Razansky
,
Chrysoula Pitsouli
,
Norbert Perrimon
,
Vasilis Ntziachristos
,
Ralph Weissleder
1
Center for Systems Biology
,
Massachusetts General Hospital
,
2
Institute for Biological and Medical Imaging (IBMI)
,
Technical University of Munich and Helmholtz Center Munich
,
3
Department of Genetics
,
Harvard Medical School and Howard Hughes Medical Institute
Chapters
00:05
Title
00:54
Introduction
01:34
Preparation of Drosophila
04:13
Mounting Drosophila Pupae for Imaging
05:16
Scanning a Tissue of Interest
06:18
Time-lapse Imaging
07:58
Three Dimensional Reconstruction of Fluorescence
09:59
Representative Results/ Outcome
10:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
介观荧光断层超越组织切片荧光显微镜渗透限制。该技术是基于多投影照明和光子运输说明。我们表现出体内的形态发生全身三维可视化的GFP表达翼成虫光盘<em>果蝇。</em
Tags
Mesoscopic Fluorescence Tomography
In-vivo Imaging
Developing Drosophila
Optical Interrogation
Molecular Changes
Functional Changes
Optical Imaging Methods
Penetration Limits
Optical Microscopy
Diffusion-imposed Limits
Optical Macroscopy
Model Organisms
Insects
Animal Embryos
Small Animal Extremities
Nanometer-resolution Optical Imaging
Imaging Penetration Depth
Photon Scattering
Entire Embryos Imaging
Chemical Treatment
Scattering Clearance
Post-mortem Imaging
Two-photon Microscopy
Confocal Microscopy
Developmental Biology
Drug Discovery
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