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汇集人类血小板裂解液作为一种有效的补充(pHPL)的动物血清自由人干细胞培养制备
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JoVE Journal
Biology
Preparation of Pooled Human Platelet Lysate (pHPL) as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
Please note that all translations are automatically generated.
Click here for the English version.
汇集人类血小板裂解液作为一种有效的补充(pHPL)的动物血清自由人干细胞培养制备
DOI:
10.3791/1523-v
•
08:32 min
•
October 30, 2009
•
Katharina Schallmoser
,
Dirk Strunk
1
Stem Cell Research Unit
,
Medical University of Graz, Austria
Chapters
00:00
Title
00:55
Introduction
01:32
Preparation of Human Platelet Lysate
06:36
Characteristics of the Resulting Lysate
08:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
人类血小板裂解物源丰富的生长因子和细胞培养的有力补充。该协议提出,准备从富含血小板血浆,执行几个冻融循环和消耗血小板片段大池的人血小板裂解过程。
Tags
Pooled Human Platelet Lysate
PHPL
Animal Serum-free
Stem Cell Cultures
Platelet Derived Growth Factors
Cell Proliferation
Mesenchymal Stromal Cells
Fibroblasts
Endothelial Colony-forming Cells
Thrombin Activation
Freeze/thaw Cycles
Platelet Releasate
Trophic Effect
Regenerative Therapy
Growth Factor Concentrations
Standardized Protocols
Platelet Preparation
Human Platelet Lysate
Platelet Rich Plasma
Freeze/thaw Cycles
Centrifugation
Aggregate Formation
Potential Antigens
Culture Protocols
Cell Therapeutics
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