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组织工程细胞在三维凝胶封装

JoVE Journal
Biology

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JoVE Journal Biology

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Please note that all translations are automatically generated. Click here for the English version.

组织工程细胞在三维凝胶封装

DOI:

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09:37 min

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October 26, 2009

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Sudhir Khetan, Jason Burdick

¹Department of Bioengineering,University of Pennsylvania , ²Department of Bioengineering,University of Pennsylvania-School of Medicine

Chapters

00:00Title
00:35Introduction
00:58Material Preparation and Sterilization
02:45Cell Preparation
04:56Hydrogel Formation
06:25Alternative Sequential Hydrogel Crosslinking
07:17Encapsulating Cells in 3D Gels Outcome and Applications
09:08Conclusion

Summary

Automatic Translation

我们目前合成水凝胶内的细胞的三维（3D）封装的协议。封装过程概述了交联（迈克尔型除了和光发起的自由基机制）两种常用的方法，以及评估封装细胞行为的一些技巧。

Tags

Cellular Encapsulation 3D Hydrogels Cell Culture Synthetic Polymeric Hydrogels Poly(ethylene Glycol)Hyaluronic Acid Reactive Groups Crosslinking Microenvironments Biochemical Cues Stem Cells Cytocompatibility Hydrogel Processing

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