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Chapters
- 00:00Title
- 00:58Introduction
- 01:31Selecting and Washing Larvae
- 02:27Dissecting the Larvae and Removing Non-Specific Cells
- 04:47Dissociating the Tissue into a Single Cell Suspension
- 08:07Magnetic Bead Cell Sorting
- 10:05Representative Results of DA Neurons Isolation and RNA Extraction
- 11:15Conclusion
In diesem Video-Artikel präsentieren wir ein Verfahren zur Isolierung und Aufreinigung von<em> Drosophila</em> Peripheren Neuronen mit einem schnellen Magnetic Bead Zellsorting Strategie. RNA aus den isolierten Zellen erhalten kann ohne weiteres für nachgelagerte Anwendungen einschließlich Microarray-Analysen verwendet werden.
Tags
IsolationPurificationDrosophila Peripheral NeuronsMagnetic Bead SortingPNS NeuronsDendritic Arborization (da) NeuronsNeuronal DevelopmentDendrite MorphogenesisFunctional And Molecular LevelsPure PopulationLow OccurrenceTough Chitinous Larval CuticleSpecific Cell EnrichmentAntibody-coated Magnetic BeadsAge-matched Third Instar LarvaeMouse CD8 Tagged GFP Fusion Protein (UAS-mCD8-GFP)Pickpocket (ppk)-GAL4 DriverPan-da Neuron-specific GAL421-7 DriverLarval CuticleCell Types
