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通过双光子荧光恢复漂白后测量扩散系数
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Biology
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JoVE Journal Biology
Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
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通过双光子荧光恢复漂白后测量扩散系数

DOI:
10.3791/1636-v

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07:00 min

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February 26, 2010

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,
1Department of Physics and Astronomy,University of Rochester, 2Department of Biomedical Engineering,University of Rochester

Chapters

  • 00:00Title
  • 00:46Introduction
  • 01:09Aligning the Optics
  • 02:51Determining Safe Monitor Powers
  • 03:54Determinging the Input Parameters to the Scaler and Pulse Generator
  • 06:00Representative Results
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

在这篇文章中，我们将描述测量扩散系数，使用多光子荧光漂白后恢复的过程。我们将开始通过调整样品沿光路的激光，并确定适当的实验参数，然后继续产生，最后装修荧光恢复曲线。

Tags

Diffusion CoefficientsTwo-photon Fluorescence Recovery After PhotobleachingMulti-fluorescence Recovery After PhotobleachingMicroscopy TechniqueMacromoleculesIn VitroIn VivoBiological SystemsPhotobleachingLaser FlashFluorescence MonitoringPockels CellLaser ModulatorOptical PathSample PreparationAqueous Fluorescent DyeExperimental ParametersMonitor PowerBleaching PowerBleach DurationBin WidthsFluorescence Recovery TimeRecovery CurvesLabVIEW AutomationEnhanced ThroughputMathematical Model Fitting Algorithm

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