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光镜研究沉默突触的突触前
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JoVE Journal Biology
Presynaptically Silent Synapses Studied with Light Microscopy
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光镜研究沉默突触的突触前

DOI:
10.3791/1676-v

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11:02 min

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January 04, 2010

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, , , ,
1Department of Psychiatry,Washington University School of Medicine, 2Department of Anatomy,Washington University School of Medicine, 3Department of Neurobiology,Washington University School of Medicine

Chapters

  • 00:00Title
  • 00:40Introduction
  • 01:08Preparation of Hippocampal Cultures
  • 03:12Immunostaining of Silent Synapses
  • 06:56Visualization of Silent Synapses
  • 09:20Representative Silent Synapse Data
  • 10:21Conclusion

Summary

Automatic Translation

Automatic Translation

谷氨酸突触主动模式可以切换到无声模式。我们证明，在啮齿类动物神经细胞的分离文化突触前的活动状态的可视化使用一个可修复的FM1 -​​ 43染料形式活跃的突触形象化vGluT - 1抗体免疫可视化所有谷氨酸突触。

Tags

PresynapticSilent SynapsesLight MicroscopySynaptic PlasticityNervous SystemLearnRememberAdaptabilityNeurotoxicPresynaptic PlasticityDigital SwitchingRelease VesiclesGlutamate NeurotransmitterVisualizing Activity StatusDissociated Cell CulturesRodent HippocampusStaining ProfileStyryl Dye FM1-43Synaptic VesiclesImmunostainingVesicular Glutamate Transporter 1 (vGluT-1)Depolarizing StimuliPresynaptic SilencingElectrical SilencingCAMP Signaling Pathways

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