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Sinapses pré-sináptico Silencioso Estudou com Microscopia de Luz

Article DOI: 10.3791/1676 • 11:03 min
January 4th, 2010 •
1, 1, 1, 1, 1,2,3
1Department of Psychiatry, Washington University School of Medicine, 2Department of Anatomy, Washington University School of Medicine, 3Department of Neurobiology, Washington University School of Medicine

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January 4th, 2010

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Sinapses glutamatérgicas pode mudar de um modo ativo de um modo silencioso. Nós demonstramos que o status de atividade pré-sináptica da cultura dissociada dos neurônios dos roedores é visualizado através de um formulário fixable do FM1-43 corante para visualizar sinapses ativas e imunocoloração com vGluT-1 anticorpos para visualizar todas as sinapses de glutamato.

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