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使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
DOI:

10:34 min

April 14, 2010

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Chapters

  • 00:00Title
  • 00:49Intro
  • 01:16Preparing and Counting the Namalwa Cells
  • 04:17Electroporating the Cells in 96-well Plates
  • 05:55Extract and Check RNA
  • 07:54Real-time PCR
  • 08:58Analysis of Gene Expression
  • 10:03Conclusion

Summary

Automatic Translation

此过程介绍了一个快速简便的工作流程,引入困难的siRNA转染细胞系,并按照实时PCR基因表达。使用自动细胞计数器,多井的电板,全自动电泳站提供快速和可靠的结果,而不需要昂贵的机械手。

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