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JoVE Journal
Biology

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使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
 
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使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒

Article DOI: 10.3791/1904-v 10:35 min April 14th, 2010
April 14th, 2010

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Summary

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Click here for the English version.

此过程介绍了一个快速简便的工作流程,引入困难的siRNA转染细胞系,并按照实时PCR基因表达。使用自动细胞计数器,多井的电板,全自动电泳站提供快速和可靠的结果,而不需要昂贵的机械手。

Tags

细胞生物学,第38期,细胞计数,基因沉默的siRNA,Namalwa细胞,IL - 4基因的表达,电,实时PCR
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