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调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em
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JoVE Journal Biology
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
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调查组织和器官特定的光敏色素反应，使用流式细胞仪辅助细胞类型特定的表达谱拟南芥</em

DOI:
10.3791/1925-v

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10:10 min

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May 29, 2010

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,
1Department of Energy – Plant Research Laboratory,Michigan State University (MSU), 2Department of Biochemistry and Molecular Biology,Michigan State University (MSU)

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:22Producing the GAL4 Enhancer-trap Line
  • 02:37Plant Growth and Leaf Protoplasts Isolation
  • 07:18Protoplast Sorting by Fluorescence-Activated Cell Sorting (FACS)
  • 08:47Leaf Protoplasts Isolation and Sorting by FACS Representative Results
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

空间特定的光敏反应的分子基础正在调查中使用，表现出组织和器官特异性的光敏色素的不足的转基因植株。参展隔离特定的细胞芯片分析排序，是用来识别在特定空间的光敏反应有关的基因的荧光激活细胞诱导的光敏色素生色枯竭。

Tags

FACS-assisted Cell-type Specific Expression ProfilingArabidopsis ThalianaPhotoreceptorsRed/far-red Light-absorbing PhytochromePlant SystemsPhytochrome FunctionsMutational AnalysesPhotoperceptionPhotomorphogenesisDownstream Target GenesCell-to-cell Signaling

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