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制备小鼠脑组织中的免疫电镜
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
制备小鼠脑组织中的免疫电镜
DOI:
10.3791/2021-v
•
08:47 min
•
July 20, 2010
•
Marie-Eve Tremblay
,
Mustapha Riad
,
Ania Majewska
1
Department of Neurobiology and Anatomy
,
University of Rochester
,
2
Douglas Mental Health University Institute
Chapters
00:08
Title
00:45
Introduction
01:14
Post-fixing and Sectioning of Brain
01:55
Pre-Embedding Immunocytochemistry
03:45
Processing for Electron Microscopy
07:36
Representative Images
08:20
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述transcardiac灌注小鼠,拆除和切片的大脑,以及免疫过氧化物酶染色,树脂包埋,和脑切片切片超薄协议。这些程序完成后,免疫染色材料透射电子显微镜检查的准备。
Tags
Immunoelectron Microscopy
Transmission Electron Microscopy
TEM
Microglial
Oligodendrocytic
Astrocytic
Neuronal Subcellular Compartments
Dendrite
Dendritic Spine
Axon
Axon Terminal
Perikaryon
Intracellular Organelles
Cytoskeleton
Pre-embedding Immunocytochemistry
Antibody
Iba1 Marker
Neurotransmitter Contents
Ultrastructural Localization Of Proteins
Acrolein Fixative
Transcardiac Perfusion
Brain Sectioning
Immunoperoxidase-diaminobenzidine Staining
Resin Embedding
Ultrathin Sectioning
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