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Method for the Isolation of Francisella tularensis Outer Membranes
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Method for the Isolation of Francisella tularensis Outer Membranes

Method for the Isolation of Francisella tularensis Outer Membranes

DOI:
10.3791/2044-v

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11:06 min

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June 29, 2010

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, ,
1Department of Microbiology,University of Texas Southwestern Medical Center

Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:40Spheroplasting and Osmotic Lysis
  • 05:48Resuspending the Membrane Pellet
  • 07:58Sucrose Density Gradient Centrifugation
  • 09:51Representative Results of Outer Membrane Isolation from F. tularensis
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for separating inner and outer membranes from Francisella tularensis by spheroplasting, osmotic lysis, and sucrose density gradient ultracentrifugation.

Tags

IsolationFrancisella TularensisOuter MembranesGram-negativeCoccobacillusZoonotic DiseaseTularemiaInfectious DoseVirulence FactorsOuter Membrane Proteins (OMPs)Host Cell BindingEntryIntracellular SurvivalVirulenceImmune EvasionProtective VaccinesGram-negative BacteriaBulk Membrane Extraction TechniquesSonicationCentrifugationDetergent ExtractionPeriplasmic ContaminantsCytoplasmic ContaminantsInner Membrane (IM)SpheroplastingOsmotic LysisSucrose Density Gradient CentrifugationLipopolys

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