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の分離のための方法野兎病菌外膜
JoVE Journal
Biology
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JoVE Journal Biology
Method for the Isolation of Francisella tularensis Outer Membranes
DOI:

11:06 min

June 29, 2010

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Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:40Spheroplasting and Osmotic Lysis
  • 05:48Resuspending the Membrane Pellet
  • 07:58Sucrose Density Gradient Centrifugation
  • 09:51Representative Results of Outer Membrane Isolation from F. tularensis
  • 10:24Conclusion

Summary

Automatic Translation

から内側と外側の膜を分離するためのプロトコル<em>野兎病菌</em> spheroplasting、浸透圧溶解、およびショ糖密度勾配超遠心分離によって。

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