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隔离方法弗朗西斯tularensis外膜
JoVE Journal
Biology
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JoVE Journal Biology
Method for the Isolation of Francisella tularensis Outer Membranes
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隔离方法弗朗西斯tularensis外膜

DOI:
10.3791/2044-v

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11:06 min

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June 29, 2010

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, ,
1Department of Microbiology,University of Texas Southwestern Medical Center

Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:40Spheroplasting and Osmotic Lysis
  • 05:48Resuspending the Membrane Pellet
  • 07:58Sucrose Density Gradient Centrifugation
  • 09:51Representative Results of Outer Membrane Isolation from F. tularensis
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

内层和外层膜分离议定书<em>弗朗西斯tularensis</em> spheroplasting，渗透溶解，和蔗糖密度梯度离心。

Tags

IsolationFrancisella TularensisOuter MembranesGram-negativeCoccobacillusZoonotic DiseaseTularemiaInfectious DoseVirulence FactorsOuter Membrane Proteins (OMPs)Host Cell BindingEntryIntracellular SurvivalVirulenceImmune EvasionProtective VaccinesGram-negative BacteriaBulk Membrane Extraction TechniquesSonicationCentrifugationDetergent ExtractionPeriplasmic ContaminantsCytoplasmic ContaminantsInner Membrane (IM)SpheroplastingOsmotic LysisSucrose Density Gradient CentrifugationLipopolys

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