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Journal
/
Biology
/
の分離のための方法
野兎病菌
外膜
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Method for the Isolation of
Francisella tularensis
Outer Membranes
Please note that all translations are automatically generated.
Click here for the English version.
の分離のための方法
野兎病菌
外膜
DOI:
10.3791/2044-v
•
11:06 min
•
June 29, 2010
•
Jason F. Huntley
,
Gregory T. Robertson
,
Michael V. Norgard
1
Department of Microbiology
,
University of Texas Southwestern Medical Center
Chapters
00:00
Title
01:05
Introduction
01:40
Spheroplasting and Osmotic Lysis
05:48
Resuspending the Membrane Pellet
07:58
Sucrose Density Gradient Centrifugation
09:51
Representative Results of Outer Membrane Isolation from F. tularensis
10:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
から内側と外側の膜を分離するためのプロトコル<em>野兎病菌</em> spheroplasting、浸透圧溶解、およびショ糖密度勾配超遠心分離によって。
Tags
Isolation
Francisella Tularensis
Outer Membranes
Gram-negative
Coccobacillus
Zoonotic Disease
Tularemia
Infectious Dose
Virulence Factors
Outer Membrane Proteins (OMPs)
Host Cell Binding
Entry
Intracellular Survival
Virulence
Immune Evasion
Protective Vaccines
Gram-negative Bacteria
Bulk Membrane Extraction Techniques
Sonication
Centrifugation
Detergent Extraction
Periplasmic Contaminants
Cytoplasmic Contaminants
Inner Membrane (IM)
Spheroplasting
Osmotic Lysis
Sucrose Density Gradient Centrifugation
Lipopolys
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