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1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, 3Department of Pathology,The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children’s Research Institute,Royal Children’s Hospital, 5Department of Pediatrics,The University of Melbourne
Chapters
- 00:00Title
- 00:05Introduction
- 01:42Tissue Preparation
- 02:29Immunofluorescence Staining
- 05:19Microscopy
- 07:02Analysis
- 08:24Conclusion
Количественный анализ ДНК двунитевых разрывов на основе γH2AX очагов стал бесценным инструментом, особенно в области радиационной биологии, для оценки радиочувствительности тканей и воздействию радиации изменения соединений. Здесь мы демонстрируем использование иммунофлуоресценции для количественного определения γH2AX очагов в образцах тканей.
Tags
QuantitationγH2AX FociDNA Double-strand BreaksEndogenous ProcessesExogenous FactorsIonizing RadiationRadiomimetic CompoundsH2A Histone VariantSerine-139 ResiduePhosphorylationPI3K Family Of ProteinsATM KinaseATR KinaseNuclear γH2AX FociImmunofluorescence MicroscopyMarker Specificity And SensitivityBiomedical ResearchRadiation BiologyNuclear MedicineCell Culture SystemsIonizing Radiation-induced DSBsCellular RadiosensitivityRadiation-modifying CompoundsDSB-inducing Compounds
