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成像蛋白质 - 蛋白质相互作用
在体内</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Imaging Protein-protein Interactions
in vivo
Please note that all translations are automatically generated.
Click here for the English version.
成像蛋白质 - 蛋白质相互作用
在体内</em
DOI:
10.3791/2149-v
•
11:15 min
•
October 10, 2010
•
Tom Seegar
,
William Barton
1
Biochemistry and Molecular Biology
,
Virginia Commonwealth University
Chapters
00:00
Title
01:10
Introduction
01:35
Construction of CFP and YFP Chimeric Receptors and Transfection of EA.hy 926 Cells
04:44
Confocal Imaging and Live FRET Analysis
08:41
Representative Results
10:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了如何形象蛋白质相互作用使用基于FRET的接近检测。
Tags
Protein-protein Interactions
In Vivo
Imaging
Genetic Encodable Fluorescent Proteins
GFP
RFP
FRET
Proximity Assay
Receptor-receptor Interactions
Endothelial Cell Surface
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