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Biology
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瓣膜内皮细胞的分离
JoVE Journal
Biology
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JoVE Journal
Biology
Isolation of Valvular Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
瓣膜内皮细胞的分离
DOI:
10.3791/2158-v
•
11:04 min
•
December 29, 2010
•
Russell A. Gould
,
Jonathan T. Butcher
1
Department of Biomedical Engineering
,
Cornell University
Chapters
00:05
Title
01:13
Introduction
01:42
Isolation of Heart Valve Leaflets
03:03
Isolation of Endothelial Layer
05:22
Isolation of Side Specific Endothelial Layer
08:03
Isolation of Interstitial Cells
09:07
Isolation of Valvular Endothelial Cells Representative Images
10:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提供了隔离和培养心脏瓣膜内皮细胞(VEC)的纯种群的方法。血管内皮细胞可以分离出了风口浪尖或单张两侧及紧随,底层的间质细胞(VIC)的隔离很简单。
Tags
Isolation
Valvular Endothelial Cells
Heart Valves
Unidirectional Blood Flow
Cardiovascular System
Mechanical Stresses
Valvular Endothelial Cells
Interstitial Cells
Repair And Remodel
In Vitro Experimentation
Isolation And Culture
Direct Isolation
Side Specific Isolation
Culture
Verification Of Pure Populations
Enzymatic Digestion
Gentle Swab Scraping Technique
Surface Cells
Centrifuged Into A Pellet
Resuspended And Plated
Collagen I Matrix
VEC Phenotype
Contact Inhibited Growth
Endothelial Specific Markers (PECAM1/CD31)
Von Willebrand Factor (vWF)
Alpha-smooth Muscle Actin (α-SMA)
Functional Characteristics
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