/

/

维持细胞培养基的Wolbachia

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Maintaining Wolbachia in Cell-free Medium

Please note that all translations are automatically generated. Click here for the English version.

维持细胞培养基的Wolbachia

DOI:

•

13:33 min

•

July 04, 2007

•

Courtney Gamston, Jason Rasgon

¹Malaria Research Institute, Bloomberg School of Public Health,Johns Hopkins University

Chapters

00:06Introduction
01:24Lysing Infected Anopheles Cells
03:34Filtering Lysates and Isolation of Wolbacchia
09:31Assay of Wolbacchia Viability Using Live/Dead Stain
12:11Conclusion
13:22End Credits

Summary

Automatic Translation

本视频介绍了净化按蚊细胞系的Wolbachia pipientis，然后在无细胞培养基培养的共生细菌的方法。细菌的生存能力的检测证明。

Tags

Wolbachia Cell-free Medium Video Protocol Purify Symbionts Cultured Mosquito Cells Fluorescent Assay Viability Extracellular Phase Replicate Eukaryotic Cells Micro-injection DNA Extraction

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在果蝇的Wolbachia细菌感染

人口控制媒介种群和遗传的驱动器使用的Wolbachia pipientis替换策略

测定蛋白酶体降解的植物无细胞系统

骨条件培养基的制备和生物测定

基于海拉细胞自由表达系统中的表达<em>疟原虫</em>棒状体蛋白

无细胞化验，研究染色质解聚在有丝分裂的结束

在大鼠提睾肌的小动脉的可视化和量化的无细胞层

维护伊蚊蚊蚊子感染沃尔巴克

中通量筛选法评估对精子中 ca^{2 +}信号和反应的影响

快速、经济、简单地生产无细菌细胞裂解物

Read Article